dopamine production in hairy root cultures of portulaca oleracea (purslane) using agrobacterium rhizogenes
نویسندگان
چکیده
an efficient transformation system for the medicinal plant portulaca oleracea was established using agropine-type agrobacterium rhizogenes atcc15834. hairy roots were obtained directly from cotyledon leaves explants seven days after inoculation with the bacteria. the highest transformation efficiency was obtained from cotyledon leaves explants, and amounted to 53.3% within two weeks. roots grew rapidly on solid growth regulator free ½ murashige and skoog medium and demonstrated characteristics of transformed roots such as fast growth and high lateral branching. successful and stable transfer of rolb gene was illustrated by pcr using specific primers of the gene. the hairy roots showed an ability to synthesize natural and medicinal product, dopamine. elicitation of dopamine production in p. oleracea hairy roots was tested using different concentrations of methyl jasmonate (0, 100, 150, 200 μm) and salicylic acid (0, 125, 250, 500 μm), added to the hairy root cultures during the late growth phase. the results showed that the various concentrations of the methyl jasmonate significantly increased the dopamine content, but, at concentration of 100 μm, its impact was the most pronounced. salicylic acid had no significant influence on dopamine production in hairy roots of p. oleracea.
منابع مشابه
In vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...
متن کاملIn vitro production of secondary metabolite using Atropa komarovii Bline&Shal (Solanaceae) hairy root culture via Agrobacterium rhizogenes ATCC15834
Background & Aim:A new sustainable tissue-based system is presented by plant hairy roots, preserving all of the several specialized types of cell with critical roles in allowing bioactive secondary molecules to be synthesized more consistently as usual. The system is also essential for studying the production of alkaloid in culture. Experimental: The ...
متن کاملEfficient Agrobacterium Rhizogenes Mediated Hairy Root Culture System for Withanolide Production from Withania Somnifera (L.) Dunal
Withania somnifera (L.) Dunal is an important medicinal plant with many pharmaceutical and therapeutic uses ranging from immunomodulation to anticancer property. Field cultivation of W. somnifera is time consuming and it not able to meet current global demands. For commercial production of withanolides, field grown plant materials have generally been used but the quality and uniformity in the l...
متن کاملTransformation of Catalpa ovata by Agrobacterium rhizogenes and phenylethanoid glycosides production in transformed root cultures.
Transformed root cultures of Catalpa ovata were established following shoots infection with four agropine strains of Agrobacterium rhizogenes. Frequency of root formation was dependent on the bacterial strain and the presence of acetosyringone in the incubation medium. It is the first report concerning the possibility of transforming Catalpa ovata by A. rhizogenes. Both transformed and untransf...
متن کاملAgrobacterium Rhizogenes-mediated Transformation of Peganum multisectum (Maxim) Bobrov and Harmine Production in Hairy Roots
Using Agrobacterium rhizogenes due to create hairy roots is a useful method to product secondary metabolites in many medicinal plants. The transgenic hairy roots were induced from Peganum multisectum (Maxim) Bobrov a medicinally important species, by infecting leaf and stem explant with wild type Agrobacterium rhizogenes strain ATTCC 15834, which led to the induction of hairy roots from 19% of ...
متن کاملمنابع من
با ذخیره ی این منبع در منابع من، دسترسی به آن را برای استفاده های بعدی آسان تر کنید
عنوان ژورنال:
journal of agricultural science and technologyناشر: tarbiat modares university
ISSN 1680-7073
دوره 16
شماره 2 2014
میزبانی شده توسط پلتفرم ابری doprax.com
copyright © 2015-2023